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Wild-type but not mutant SOD1 transgenic astrocytes promote the efficient generation of motor neuron progenitors from mouse embryonic stem cells

Yiota A Christou1*, Kyoji Ohyama3, Marysia Placzek3, Peter N Monk2 and Pamela J Shaw1

Author Affiliations

1 Department of Neuroscience, Sheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, S10 2HQ, UK

2 Department of Infection and Immunity, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield, S10 2HQ, UK

3 MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, University of Sheffield, Sheffield, S10 2RX, UK

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BMC Neuroscience 2013, 14:126  doi:10.1186/1471-2202-14-126

Published: 17 October 2013

Additional files

Additional file 1: Figure S1:

Examination of the effect of astrocyte conditioned media on the apoptosis of motor neuron-progenitor cells and motor neurons. (a, b) The apoptotic nuclei of Olig2+ MN-progenitor cells (a) and Hb9:eGFP+ MNs (b) were counted in three independent experiments in a total of 30 random fields per condition. Statistically significant association of the number of apoptotic cells with the media was examined using Chi-square test with 95% Confidence Interval (alpha=0.05). There is no statistically significant association between the level of apoptosis of Olig2+ cells (P value 0.2787) or eGFP+ MNs (P value 0.3028) with any of the three media. Apoptotic Olig2+ cells are 3.98%, 3.22% and 4.51% of the total Olig2+ cells in Tg SOD1G93A CM, wt non-Tg CM and control medium respectively. Apoptotic eGFP+ MNs are 6.32%, 3.11% and 3.82% of the total eGFP+ MNs in Tg SOD1G93A CM, wt non-Tg CM and control medium respectively.

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