A. Concentration response curves elicited by cumulative application of ET-1 in human cerebral arteries incubated for 48 h with or without the MEK1/2 inhibitor U0126 (5 μM) and control human arteries. The responses to ET-1 are clearly increased in the incubated arteries as compared to control arteries. The enhanced contraction observed after organ culture was inhibited in arteries cultured with U0126. Data are expressed as mean ± s.e.m and n refer to the number of patients used. B and C Sections from the human cerebral artery showing ETA and ETB receptor immunoreactivity in the smooth muscle cell layer. D Expression of ETA and ETB protein levels in human cerebral arteries incubated for 48 h with or without the MEK1/2 inhibitor U0126 (5 μM) and control human arteries. Data are expressed as percentage of control and given as mean ± s.e.m. *P<0.05, ** P<0.01.
Ansar et al. BMC Neuroscience 2013 14:12 doi:10.1186/1471-2202-14-12