Number of neurons in brain/brainstem nuclei retrogradely labeled with Fluorogold injected 8 mm caudal to the thoracic spinal cord lesion site. OEG transplantation into the contused spinal cord 14 days after injury promotes significant axonal sparing/regeneration of Raphe-, Vestibular- and Reticulo-spinal axons. Graphs depict the mean numbers of total FG-labeled neurons in the brains and brainstems of the four groups (A). Mean neuron numbers of the Raphe (B), Rubrospinal (C), Hypothalamic (D), Reticular (E), Trigeminal/DC (F), Cortical layer V (G) and Vestibular (H). Experimental groups were compared using a one-way analysis of variance (ANOVA), followed by the Dunnett’s method of multiple comparisons versus a single control group (injury only) and also the Tukey test for multiple comparison procedures between all experimental and control groups.
Barbour et al. BMC Neuroscience 2013 14:106 doi:10.1186/1471-2202-14-106