Glial scar formation at the lesion site indicated by GFAP and CSPG immunoreactivity. A-D: GFAP in green DsRed2-labeled transplanted cells in red. A-B: Lesion site 2 weeks after transplantation of OEG. C-D: Lesion site 2 weeks after transplantation of Schwann cells. Dispersal of transplanted cells is greater for OEG than for Schwann cells. Schwann cells remain in a dense aggregate at the lesion site surrounded by strong GFAP immunoreactivity. E: Schwann cells are surrounded by dense proteoglycan network CSPG (green), DsRed2-labeled transplanted cells (red). Scale bars: A-D: 100 μm, E: 200 μm. F: Graph showing the quantitative fluorescence (measured by mean pixels) of GFAP and CSPG at the distal interface of the transplant zone (n=10 for each group). OEG had significantly less (p<0.05) CSPG expression when compared to Schwann cells (see *). Schwann cells had significantly greater (p< 0.05) CSPG expression when compared to medium injected controls (see #).
Barbour et al. BMC Neuroscience 2013 14:106 doi:10.1186/1471-2202-14-106