Scheme of time line of the procedure used. All rats were ovariectomized and let them recover for two weeks before any procedure was made. All experimental groups were pre-treated with 17 beta estradiol (E2) (25 μg/kg) for two weeks before A-β25–35 injection in HIPP or OB. 8 or 15 days + E2 groups received one or two weeks respectively additional injections of E2. Olfactory tests and brain extraction were carried out 24 h, 8 or 15 days after A-β25–35 injection.
Bernal-Mondragón et al. BMC Neuroscience 2013 14:104 doi:10.1186/1471-2202-14-104