Figure 4.

Oxidative stress increases aggregation of ATXN7Q65-Myc. A) HEK 293 cells transfected to express ATXN7Q10-myc or ATXN7Q65-myc was treated with 0–0.5 mM H2O2 for 48 h and ATXN7 levels were analyzed by western blot. Upper panel; a representative western blot, lower panel: quantification of ATXN7 expression levels. B) Analysis of aggregated ATXN7 in cells transfected and treated as in A. Upper panel; a representative blot, lower panel; quantification of ATXN7 aggregation. C) HEK 293 cells transfected as in A was treated with 0–2 mM BSO for 48 h and western blot performed. Upper panel; a representative western blot, lower panel; quantification of ATXN7 expression levels. D) Analysis of aggregated ATXN7 in cells treated as in C. Upper panel; a representative blot, lower panel; quantification of ATXN7 aggregation. Actin was used as loading control in all western blot experiments. All quantitative data are shown as means ± SEM from three independent experiments with triplicates. NS: not significant, * p <0.05, ** p <0.01 and *** p <0.001.

Ajayi et al. BMC Neuroscience 2012 13:86   doi:10.1186/1471-2202-13-86
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