Effect of MMPSense750 cleavagein vitro. Addition of 5 μl MMPSense750 to 95 μl blood from animals following stroke produced no significant additional increase in intensity when compared to its addition to a control solution of phosphate buffer-(PBS). In contrast, the addition of MMPSense750 to a solution of 45 μl PBS + 50 μl trypsin resulted in a marked increase in fluorescent intensity (P <0.001, 2 way ANOVA).
Barber et al. BMC Neuroscience 2012 13:76 doi:10.1186/1471-2202-13-76