Table 2

Functional properties of recombinant nAChRs expressed inXenopusoocytes
Subunits EC50(μM or nM)* Hill slope n ** Imax[Imean](nA)
Dα5 8.8 ± 2.5 μM 1.1 ± 0.3 6 200 [141 ± 25]
Dα6
Dα7 6.7 ± 1.7 μM 1.0 ± 0.3 4 86 [45 ± 13]
Dα5 + Dα6 8.6 ± 2.4 μM 1.0 ± 0.1 5 47 [20 ± 8]
Dα5 + Dα7 1.6 ± 0.3 μM 1.0 ± 0.1 3 53 [39 ± 8]
Dα6 + Dα7
Dα5 + Dα6 + Dα7 13.5 ± 1.7 nM* 1.2 ± 0.3 6 150 [107 ± 12]

Note, in all cases, nAChR subunits were co-expressed with CeRIC-3.

* Note, EC50 value for Dα5 + Dα6 + Dα7 is expressed as nM, rather than μM.

**EC50 and Hill slopes are means ± SEM of separate fits to dose–response curves derived from independent experiments (n = 3–6).

† Relatively small currents were detected with all subunit combinations, as indicated by the size of the maximum current that was detected with each subunit combination (Imax) and the mean maximum current (Imean) from between 6–20 different oocytes.

‡ Subunit combinations that failed to generate functional responses are indicated by a dash. This is based on studies conducted with at least 5 batches of oocytes that generated functional nAChRs with other subunit combinations and at least 10 oocytes from each batch (n > 50).

Lansdell et al.

Lansdell et al. BMC Neuroscience 2012 13:73   doi:10.1186/1471-2202-13-73

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