Table 1

Radioligand binding toDrosophilanAChR subunit combinations
Subunit combination [125I]-α-BTX Binding [3H]-epibatidine binding
- RIC3 + RIC3 - RIC3 + RIC3
Dα5
Dα5/Dα1
Dα5/Dα2
Dα5/Dα3
Dα5/Dα4
Dα5/Dα6 +
Dα5/Dα7
Dα5/Dβ1
Dα5/Dβ2
Dα5/Dβ3
Dα5/Dα1/Dα2
Dα5/Dα1/Dβ1
Dα5/Dα1/Dβ2
Dα5/Dα1/Dβ3
Dα5/Dα2/Dβ1
Dα5/Dα2/Dβ2
Dα5/Dα2/Dβ3
Dα5/Dα3/Dβ1
Dα5/Dα3/Dβ2
Dα5/Dα3/Dβ3
Dα5/Dα4/Dβ1
Dα5/Dα4/Dβ2
Dα5/Dα4/Dβ3
Dα5/Dα6/Dα7
Dα5/Dβ1/Dβ2
Dα5/Dβ1/Dβ3
Dα5/Dβ2/Dβ3
Dα5/Dα1/Dα2/Dβ1
Dα5/Dα1/Dα2/Dβ2
Dα5/Dα1/Dα2/Dβ3
Dα5/Dα1/Dα3/Dβ1
Dα5/Dα1/Dα3/Dβ2
Dα5/Dα1/Dα3/Dβ3
Dα5/Dα1/Dα4/Dβ1
Dα5/Dα1/Dα4/Dβ2
Dα5/Dα1/Dα4/Dβ3
Dα5/Dα2/Dα3/Dβ1
Dα5/Dα2/Dα3/Dβ2
Dα5/Dα2/Dα3/Dβ3
Dα5/Dα2/Dα4/Dβ1
Dα5/Dα2/Dα4/Dβ2
Dα5/Dα2/Dα4/Dβ3
Dα5/Dα3/Dα4/Dβ1
Dα5/Dα3/Dα4/Dβ2
Dα5/Dα3/Dα4/Dβ3
Dα5/Dα6/Dα7/Dβ1
Dα5/Dα6/Dα7/Dβ2
Dα5/Dα6/Dα7/Dβ3
Dα5/Dβ1/Dβ2/Dβ3
Dα5/Dα1/Dα2/Dα3/Dβ1
Dα5/Dα1/Dα2/Dα3/Dβ2
Dα5/Dα1/Dα2/Dα3/Dβ3
Dα5/Dα1/Dα2/Dβ1/Dβ2
Dα5/Dα1/Dα3/Dβ1/Dβ2
Dα5/Dα1/Dα4/Dβ1/Dβ2
Dα5/Dα2/Dα3/Dβ1/Dβ2
Dα5/Dα2/Dα4/Dβ1/Dβ2
Dα5/Dα3/Dα4/Dβ1/Dβ2
Dα5/Dα1/Dα2/Dβ2/Dβ3
Dα5/Dα1/Dα3/Dβ2/Dβ3
Dα5/Dα1/Dα4/Dβ2/Dβ3
Dα5/Dα1/Dβ1/Dβ2/Dβ3
Dα5/Dα2/Dα3/Dβ1/Dβ2
Dα5/Dα2/Dα4/Dβ1/Dβ2
Dα5/Dα2/Dβ1/Dβ2/Dβ3
Dα5/Dα3/Dα4/Dβ1/Dβ2
Dα5/Dα3/Dβ1/Dβ2/Dβ3
Dα5/Dα1/Dα2/Dα3/Dα4/Dβ1
Dα5/Dα1/Dα2/Dα3/Dα4/Dβ2
Dα5/Dα1/Dα2/Dα3/Dα4/Dβ3
Dα5/Dα6/Dα7/Dβ1/Dβ2/Dβ3
Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ2
Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ1/Dβ2
Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ1/Dβ2/Dβ3

To illustrate the extent of radioligand binding studies undertaken, the Table lists all subunit combinations containing Dα5 that were examined in transfected Drosophila S2 cells. Binding studies were performed with [125I]-α-bungarotoxin (10 nM) and [3 H]-epibatidine (30 nM). Combinations of Drosophila nAChR subunit cDNAs were transfected in the absence or presence of RIC-3 cDNA. Data indicating presence or absence of specific binding are derived from at least 3 independent experiments.

Lansdell et al.

Lansdell et al. BMC Neuroscience 2012 13:73   doi:10.1186/1471-2202-13-73

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