Timeline of experimental protocol. Rats self-administered methamphetamine (meth) for 14 consecutive days (3 hrs/day). Days (D) 1–7 a fixed ratio (FR) 1 schedule of reinforcement was used for training. To increase responding rates, an FR5 was employed for days 8–14. On day 15, rats were acclimated to the cue reactivity (CR) protocol. Assessments of 5-HT2C ligands on meth-seeking were made days 18, 21, 24, and 27 (i.e., CR2-CR5); treatments for CR were randomized. Between each CR test, rats were allowed to self-administer meth for two consecutive days on an FR5 schedule (3 hrs/day). Five days after CR5 (on day 33), motor assessments were initiated.
Graves and Napier BMC Neuroscience 2012 13:65 doi:10.1186/1471-2202-13-65