Open Access Highly Accessed Research article

Transcriptome analysis of amoeboid and ramified microglia isolated from the corpus callosum of rat brain

Rangarajan Parakalan1, Boran Jiang1, Baby Nimmi1, Manivannan Janani1, Manikandan Jayapal2, Jia Lu1, Samuel SW Tay1, Eng-Ang Ling1 and S T Dheen1*

1 Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Blk MD10, 4 Medical Drive, Singapore, 117597, Singapore

2 Center of Excellence in Genomic Medicine Research, King Fahd Medical Research Center, King Abdulaziz University, PO Box 80216, Jeddah, 21589, Kingdom of Saudi Arabia

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BMC Neuroscience 2012, 13:64 doi:10.1186/1471-2202-13-64

Published: 14 June 2012

Additional files

Additional file 1 :

Sheet S1. Primer sequences .

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Additional file 2 :

Figure S1. Expression of Dcx and Mbp in BV-2 microglia. PCR was done using cDNA obtained from BV-2 microglia for Dcx and Mbp. Both Dcx and Mbp were found to be expressed by BV-2 microglia as identified by the PCR products in agarose gel electrophoresis.

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Additional file 3 :

Sheet S2. AMC and RMC genes with P-value.

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Additional file 4 :

Sheet S3. AMC and RMC gene clusters.

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Additional file 5 :

Sheet S4. Genes involved in CDC42-RAC pathway for migration, Synaptic transmission and genes downregulated by the Runx1-Runx1t1 complex.

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Additional file 6 :

Sheet S5. Functions of AMC and RMC.

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Additional file 7 :

Sheet S6. Monocytic genes enriched in AMC and RMC.

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Additional file 8 :

Sheet S7. ESC, NSC and HSC genes enriched in AMC and RMC.

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Additional file 9 :

Figure S2. Pathway analysis. Novel molecular networks identified by inputting AMC and RMC expression data into Adriane Pathway Studio. Red colored shapes specify AMC genes and violet colored shapes specify RMC genes. Some AMC genes - Sox4, Sox11, Runx1t1 are highlighted in the figure.

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Additional file 10 :

Sheet S8. Expression of cytokines in AMC and RMC.

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