Table 3

Number of labeled motor and sensory neurons after ETS neurorrhaphy
ETS Motor R G Y G + Y G/Total Y/Total G + Y/Total
Plasmid phVEGF 128.8 ± 45.8 46.3 ± 19.0* 5.2 ± 0.8* 51.5 ± 19.3* 0.262 ± 0.088* 0.030 ± 0.007* 0.292 ± 0.089*
Plasmid 135.4 ± 34.3 9.6 ± 4.9 2.2 ± 0.8 11.8 ± 5.1* 0.071 ± 0.041 0.016 ± 0.008 0.087 ± 0.045
Vehiculum 161.3 ± 51.9 10.3 ± 2.1 1.5 ± 0.8 11.8 ± 1.6* 0.065 ± 0.025 0.009 ± 0.003 0.074 ± 0.024
ETS Sensory
Plasmid phVEGF 323.2 ± 88.7 48.3 ± 18.6 28.7 ± 13.1* 77.0 ± 15.9* 0.117 ± 0.094 0.091 ± 0.070* 0.208 ± 0.080*
Plasmid 337.0 ± 57.6 33.8 ± 6.7 3.0 ± 1.7 36.8 ± 7.8 0.092 ± 0.022 0.008 ± 0.004 0.099 ± 0.024
Vehiculum 386.0 ± 34.1 35.7 ± 17.4 1.7 ± 1.2 37.3 ± 18.0 0.092 ± 0.050 0.005 ± 0.004 0.097 ± 0.033

* indicates statistically significant differences compared with the plasmid or vehiculum group (p < 0.05).

The results of number of labeled motor and sensory (DRG) neurons the axons of which are present in the UN of rats after ETS neurorrhaphy of the MCN stumps transfected by plasmid phVEGF, plasmid only or treated with vehiculum (n = 6 for each group). The motor and DRG neurons labeled with red fluorescence (R) are associated with motor and sensory axons present in the donor UN without contribution to reinnervation of the MCN stump. Green fluorescence (G) indicated neurons whose axons were damaged during surgical treatment and regenerated only into the MCN distal stump. A yellow color (Y) is the labeling of neurons that resulted from a mixture of red and green fluorescence and indicated those neurons, the donor axons of which were present in the UN and had sent out collateral sprouts into the distal stump of MCN. Therefore, G + Y labeled neurons indicated the number of neurons contributing to reinnervation of the MCN stump after ETS neurorrhaphy. The relation among G, Y and G + Y labeled neurons to total number of labeled neurons was calculated.

Haninec et al.

Haninec et al. BMC Neuroscience 2012 13:57   doi:10.1186/1471-2202-13-57

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