Figure 1.

Immunofluorescence staining for VEGF. Immunofluorescence staining for VEGF in the cryostat sections through intact musculocutaneous nerve (A) and distal stump of MCN transfected with plasmid phVEGF 2 months after its end-to-end (B) and end-to-side (C) neurorrhaphy. Double immunostaining for VEGF (Alexa-488) and neurofilaments (TRITC) indicated increased VEGF staining of Schwann cells in distal stump of MCN transfected with plasmid phVEGF 2 months after its end-to-end neurorrhaphy (D). Moreover, double immunostaining for VEGF (Alexa-488) and RECA (TRITC) displayed location of VEGF protein in blood vessels of transfected MCN stump 2 months after its end-to-side neurorrhaphy (E). Arrows - Schwann cells; arrowheads - blood vessels. Scale bars for A-C = 90 μm, D = 20 μm, E = 30 μm, for insets = 3 μm

Haninec et al. BMC Neuroscience 2012 13:57   doi:10.1186/1471-2202-13-57
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