Functional expression of GAMT and AGAT in rat astrocytes. Intracellular [13 C2,15N3]GAA (A) and [13C2,15N3]Cr (B) concentrations in function of [13C2,15N]Gly and[15 N2]Arg concentrations in culture medium, in three sets of cell samples (experiment 1, 2 and 3) are reported. To test the correlation among the intracellular concentration of labelled products and the precursor concentrations in culture medium, linear regression analysis was performed. Whereas there was a linear correlation for labelled GAA (p < 0.0022), no clear and consistent linear correlation for labelled Cr was found. Both graphs show that the cultures of experiment 3 yielded a lesser amount of GAA and Cr. Inspection of the cultures did not provide any obvious explanation for this result.
Carducci et al. BMC Neuroscience 2012 13:41 doi:10.1186/1471-2202-13-41