Functional expression of CT1 in rat cerebellar granule cells and astrocytes. Incubation of rat cerebellar granule cells (A) and astrocytes (B) with 1 mmol/L D3Cr in culture medium in presence and absence of 1 mmol/L GPA: intracellular D3Cr mean values are given along with standard deviation for three different experiments (each sample was analysed two times). The addition in culture medium of GPA resulted in a marked inhibition of D3Cr uptake (77% and 68% respectively in cerebellar granule cells and astrocytes). D3Cr uptake in absence of GPA was significantly higher than that in presence of GPA, showing the occurrence of Cr active transport, in cerebellar granule cells as well as in astrocytes (paired Student's t-test, N = 6, p < 0.05). Difference in D3Cr uptake between the two cell types was not statistically significant (unpaired Student's t-test, N = 6, p = 0.17).
Carducci et al. BMC Neuroscience 2012 13:41 doi:10.1186/1471-2202-13-41