Molecular identification of GluR1, GluR2, HSP70, HSP27 and HIF-1α inProtopterus annectens. Primers sequences used for the amplification of AMPA receptor subtypes and protective factors were reported in the table (a). The partial sequences obtained in Protopterus annectens have been aligned with GluR1 protein sequences (b) of Rattus norvegicus [GenBank: CAA35050] and Oreochromis mossambicus [GenBank: AAL34309], while in the case of GluR2 (c) with those of Rattus norvegicus [GenBank: AAD51284], Carassius auratus [GenBank: CAP08035] and Danio rerio [GenBank: AAQ08956]. Similarly, HSP70 partial protein sequence of Protopterus annectens(d) was aligned to heterologs of Rattus norvegicus [GenBank: AAA17441], Danio rerio [GenBank: AAC17598] and Thalassoma pavo [GenBank: ABN58790], HSP27 (e) with those of Rattus norvegicus [GenBank: AAA41353] and Salmo salar [GenBank: ACI68354] and HIF-1α (f) with sequences of Rattus norvegicus [GenBank: AAD24413], Danio rerio [GenBank: AAI65712] and Cyprinus carpio [GenBank: ABV59209].
Giusi et al. BMC Neuroscience 2012 13:39 doi:10.1186/1471-2202-13-39