Effect of 6-OHDA on neurochemical markers in striatum. Panels A-D are photomicrographs of striatum seven days following 6-OHDA administration, showing GLT-1 immunostaining in striatum on lesioned (A) and intact (B) side and GFAP immunostaining - green on lesioned (C) and intact (D) side. Scale bar = 50 μm. E shows Western blots of striatal protein samples taken from 6-OHDA lesioned animals. Blots from two animals are shown for comparison. Membranes were blotted with antibodies against tyrosine hydroxylase (TH), GLT-1, GLAST, GFAP or beta-tubulin (βtub). Molecular weights (KDa) of protein bands are indicated, the glutamate transporters GLT-1 and GLAST are found as monomeric (67 KDa) and multimeric (ca. 190 KDa) species.
Carbone et al. BMC Neuroscience 2012 13:38 doi:10.1186/1471-2202-13-38