Table 5

Quantitative PCR results for different HTR2C primers by breed background

Relative quantity HTR2CL

Relative quantity HTR2CS


I (n = 9)

26.98 ± 3.66

51.53 ± 4.40

C(n = 9)

24.71 ± 3.21

44.03 ± 3.95

LW I (n = 4)

32.77 ± 3.10

54.37 ± 7.60

LW C (n = 4)

29.37 ± 3.70

51.92 ± 6.00

HxLW I (n = 4)

20.24 ± 2.30

45.41 ± 5.80

HxLW C (n = 4)

18.53 ± 1.0

38.30 ± 3.30


Quantitative PCR was carried out using primer pairs HTR2CL (specific for the long isoform) and primer pair HTR2CS (both isoforms) using PSMD2 primer pairs as a control described in Quilter et al. 2008. Results from all pairs (n = 9) and from Large White pairs (n = 4) or Hampshire x Large White pairs (n = 4) in Table 5 are given as the mean ± SEM. No statistically significant differences were observed between the infanticidal and control groups within breed, but the LW results were statistically different from the HxLW results (p = 0.001) for the expression of the long isoform

Quilter et al. BMC Neuroscience 2012 13:37   doi:10.1186/1471-2202-13-37

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