Effects of indomethacin and β-bungarotoxin on TTX-sensitive currents in cerebellar neurons. 1A: Example of TTX-sensitive currents: currents were activated by depolarization of cells from a holding potential of -70 mV in nine voltage-steps with 10 mV increasing amplitude and 50 ms duration; currents were measured in the presence and in the absence of TTX (10 nM). Currents measured in before application of TTX were subtracted from currents measured in the presence of TTX and used for further analysis. 1B: Effect of indomethacin (10 μM): currents were stimulated by a voltage-step from -70 mV to -10 mV and plotted as relative current to the maximal current amplitude. Indomethacin led to a faster time-dependent activation. 1C: Effect of indomethacin on TTX-sensitive Na+ currents: current density was plotted against the potentials of the electrical stimulation. Indomethcin led to increased current density. 1D: Effect of β-Bungarotoxin (β-BuTX, 10 pM) on TTX-sensitive currents. Currents were normalized to the membrane capacitance and plotted as current density against the potentials of the electrical stimulation. 1E: Inhibition of β-BuTX effects by indomethacin: currents were measured in the presence of either β-BuTX or in the presence of β-BuTX together with indomethacin; current density was calculated and plotted against the potentials of the electrical stimulation. (* p < 0.05).
Guo et al. BMC Neuroscience 2012 13:36 doi:10.1186/1471-2202-13-36