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Open Access Research article

Modulation of TTX-sensitive voltage-dependent Na+ channels by β-bungarotoxin in rat cerebellar neurons

Da Guo1, Wei Xiang2, Angela Seebahn2, Cord-Michael Becker2 and Olaf Strauß1*

Author Affiliations

1 Experimental Ophthalmology, Eye Hospital, University Medical Center Regensburg, Franz-Josef-Strauss Allee 11, 93053 Regensburg, Germany

2 Institute of Biochemistry, Emil-Fischer Zentrum, Friedrich-Alexander University Erlangen-Nürnberg, Fahrstraße 17, 91054 Erlangen, Germany

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BMC Neuroscience 2012, 13:36  doi:10.1186/1471-2202-13-36

Published: 29 March 2012

Additional files

Additional file 1:

Figure S1 Isolation of TTX-sensitive currents. A: Electrical stimulation: The cells were electrically stimulated by 10 depolarizing voltage-steps of 50 ms duration with 10 mV increasing amplitude from a holding potential of -70 mV. B: Whole-cell currents measured under K+-free conditions: Voltage-dependent currents elicited by the electrical stimulation shown in A. C: Voltage-dependent currents in the presence of 10 nM TTX: Whole-cell currents from the same cell as shown B. D: TTX-sensitive currents resulting from subtraction of the currents shown in B and C.

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Additional file 2:

Figure S2 Effects of β-BuTX on voltage-dependent Ca2+ channels. A: Currents through voltage-dependent Ca2+ channels were measured under extra- and intracellular K+-free conditions in the presence of 10 mM Ba2+ as a charge carrier and 10 nM TTX to block Na+ channel currents. The cells were electrically stimulated by 10 depolarizing voltage-steps of 50 ms duration with 10 mV increasing amplitude from a holding potential of -70 mV. B: In the presence of Ba2+ the electrical stimulation results in fast activating and slowly inactivating inward currents. C: Current/voltage plot showing the effects of 10 pM β-BuTX on Ba2+ currents. The currents activated at -40 mV and reached maximal current amplitude at 0 mV. The currents were inhibited by 20% after application of 10 μM (+)BayK8644 indicting that several types of high-voltage activated Ca2+ channels contribute to the Ba2+ currents. Application of β-BuTX did change neither current density nor voltage-dependence of the Ba2+ currents.

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