Immunohistochemical staining of organotypic spinal cord co-cultured with peripheral nerve graft. Co-cultures were incubated for one week with 100 μg/ml green fluorescent MNPs and were stained with anti-pan-neuronal neurofilament to visualize neurons, shown in images (A) and (B). Neurons do not display co-localization with green fluorescent MNPs. Microglia in (C) and (D) were visualized with the microglial marker anti-CD11b/c and demonstrated high amount of MNPs localized in vesicles. Astroglial cells in (E) and (F) were stained with anti-GFAP antibody and showed moderate MNP co localization (arrows). Bars in (A) and (E) represent 75 μm, in (C) 50 μm and in (B), (D) and (F) 25 μm.
Pinkernelle et al. BMC Neuroscience 2012 13:32 doi:10.1186/1471-2202-13-32