Figure 1.

Overexpression of PHP in cholinergic neurons causes cell damage. (A) Western blot analysis shows overexpression of PHP and of its inactive mutant in SN56 cells after 48 h. An amount of 100 ng recombinant PHP and 50 μg total cell lysate were applied for SDS-PAGE. PHP was detected by the monoclonal PHP antibody. As a loading control α-tubulin was used and detected by the monoclonal α-tubulin antibody. (B) Percentage of damaged SN56 cells was determined by Hoechst 33258 staining and was increased after PHP overexpression. However, cell damage was less pronounced after overexpression of the H53A-PHP mutant. Values are given as means ± standard deviationof 6 experiments. ***p < 0.001 and **p < 0.01 compared to GFP-overexpression; ##p < 0.01 compared to H53A-overexpression (ANOVA followed by Scheffé-test)

Eißing et al. BMC Neuroscience 2012 13:31   doi:10.1186/1471-2202-13-31
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