Additional file 3.

Figure 3. SLPI treatment of human naïve CD4+ T cell culture does not influence differentiation of Th1 and Th17 cells. A: Human naïve CD4+ T cells were stimulated with anti-CD3- and anti-CD28-coated beads in RPMI medium in the absence or presence of 500 ng/mL SLPI. After four days, the cells were activated with PMA, Ionomycin and Brefeldin A for 4 h and analyzed by flow cytometry for CD4 (DAPI channel), IFN-g (Pe-Cy7 channel) and IL-17 (FITC channel). Left side: Representative dot blot of control treated naïve human CD4+ T cell cultures, right side: representative dot blot of T cell cultures incubated with 500 ng/mL SLPI. B: The column chart shows the average concentration of IFNg + CD4+ T cells in the CD4+ T cell cultures. p = 0.11 comparing numbers of IFNg + CD4+ T cells incubated with 500 ng/ml SLPI and without SLPI. A respective diagram showing the average number of Th17 cells is not shown due to the lack of these cells in our T cell cultures.

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Müller et al. BMC Neuroscience 2012 13:30   doi:10.1186/1471-2202-13-30