Manipulation of neuronal DA levels after the larval CNS has developed has no effect on feeding behavior or gut fiber architecture. Control - uninduced; RU486 - induced. A. Feeding. B. Locomotion. n = 40 for behavioral analyses, from 4-6 independent experiments. C. Number of branches per 0.1 mm length along the axonal fiber. D. Number of large varicosities per 0.1 mm length. GSelav/THA;THK uninduced control, 41 fibers from 34 guts from 7 independent experiments; GSelav/THA;THK induced (RU486), 41 fibers from 33 guts from 8 independent experiments; GSelav/THUAS27.6 uninduced control, 40 fibers from 30 guts from 6 independent experiments; GSelav/THUAS27.6 induced (RU486), 39 fibers from 28 guts from 9 independent experiments. Statistical analyses were performed using Student's t test comparing uninduced with induced animals for each genotype. Lines above the graph depict standard error of the mean.
Neckameyer and Bhatt BMC Neuroscience 2012 13:26 doi:10.1186/1471-2202-13-26