Effect of specific antagonists on Cai++ release. Cells were plated at 16,000 cells/well for 24 hrs, and loaded with Calcium 4 dye reagent. A. 5-HT2AR- or 5-HT2CR-CHO cells were preincubated with M100907 or SB242084, respectively, for 15 min then stimulated with 1 μM 5-HT. B. 5-HT2CR-CHO cells were preincubated with vehicle or 1 μM SB216641 for 15 min then stimulated with varying concentrations of 5-HT. Inset. Parental CHO-K1 cells (no preincubation) were treated with varying concentrations of 5-HT. n = 3-4 per group.
Seitz et al. BMC Neuroscience 2012 13:25 doi:10.1186/1471-2202-13-25