Coomassie stained gel of proteins isolated from exosomal fractions derived from primary cortical neurons. Primary cortical neurons (2500000 cells) were treated with or without Wnt3a (10 nM) for 8 hours in SFM. Cell culture medium was collected, centrifuged at 10000xg for 10 minutes, concentrated using 3kDa centrifugal devices and exosomes were isolated at 100000xg. Proteins were separated by SDS-PAGE and visualised using coomassie blue. Western blot of Wnt3a and Alix in exosomal fractions derived from primary cortical neurons treated with and without Wnt3a in SFM for 8 hours (B).
Hooper et al. BMC Neuroscience 2012 13:144 doi:10.1186/1471-2202-13-144