Open Access Highly Accessed Research article

Visualization of odor-induced neuronal activity by immediate early gene expression

Asim K Bepari12, Keisuke Watanabe2, Masahiro Yamaguchi3, Nobuaki Tamamaki1 and Hirohide Takebayashi24*

Author Affiliations

1 Department of Morphological Neural Science, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan

2 Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan

3 Department of Physiology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan

4 PRESTO, Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan

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BMC Neuroscience 2012, 13:140  doi:10.1186/1471-2202-13-140

Published: 5 November 2012

Additional files

Additional file 1:

Figure S1. Strong residual activity at the necklace glomeruli in Cnga2-null mice. Figure shows horizontal sections of the OB. Expression of Th, a marker of afferent activity, was significantly reduced in most of the OB glomeruli in Cnga2-null mice (B1) compared to that of wild type mice (A). However, strong Th expression was observed in a small number of glomeruli (B1, inset), presumably the necklace glomeruli which express Pde2 (B2, inset). In Cnga2-null mice c-fos expression was almost absent in the OB. However, strong c-fos signals appeared in a few glomeruli (B3, inset). Scale bar: 200 μm.

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Additional file 2:

Movie S1. Behavioral responses of wild type and Cnga2-null male mice after presentation of estrous female mice.

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Additional file 3:

Movie S2. Positive sexual behavior in Cnga2-null male mice.

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Additional file 4:

Movie S3. TMT-induced avoidance test.

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Additional file 5:

Table S1. Information on ISH probes.

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