Figure 3.

Histological analysis and quantification of the absolute numbers of co-localization of BrdU-positive cells with NG2+cells, CNPase+mature oligodendrocytes and nestin-GFP+neural precursors in the SN. Effects of 10 days of levodopa treatment. A: In short term groups MPTP induced a significant increase in the number of co-labeled BrdU+ and NG2+ in the SN compared to saline-treated controls. At later time points this effect was not detectable. The numbers of co-labeled BrdU+ and NG2+ cells decreased over time in controls and MPTP groups. B: At day 10 the number of co-labeled BrdU+ and NG2+ cells was significantly increased in MPTP mice that were treated with levodopa. C: After 3 days no newborn mature oligodendrocytes were detectable. In 28- and 70 day groups MPTP induced a decrease in co-labeled BrdU+ and CNPase+ cells compared to saline treated controls where an increase in CNPase+ cells was observed between 10 and 28 days. D: At day 10 the number of BrdU+-labeled mature oligodendrocytes was not influenced by MPTP or levodopa. There was no significant interaction between levodopa treatment and MPTP-treatment. E: In short term groups MPTP induced a significant increase in the number of BrdU+ neural precursors (Nes-GFP-positive, Nes-GFP+) in the SN compared to saline-treated controls. In later groups no such effect was detectable. The number of newborn NesGFP+ cells decreased over time in MPTP groups. F: At day 10 the number of BrdU+ neural precursors was not influenced by MPTP or levodopa. There was no significant interaction between levodopa treatment and MPTP-treatment. *p < 0.05, **p < 0.001 versus corresponding controls, ┌*┐p < 0.05, ┌**┐p < 0.001 versus other groups (n ≥ 5).

Klaissle et al. BMC Neuroscience 2012 13:132   doi:10.1186/1471-2202-13-132
Download authors' original image