mRNA, protein and activity of brain iPLA2and sPLA2in rats fed the control or high-sucrose diet for 8 weeks. iPLA2 (VIA) A) mRNA, B) protein and C) activity; sPLA2 (IIA) E) mRNA, F) protein and G) activity. Data are expressed as the relative level of the iPLA2 and sPLA2 normalized to the endogenous control (β-globulin) using the ΔΔCT method. Protein level is the ratio of optical density of iPLA2 and sPLA2 to β–actin, expressed as percent of control. Values are mean ± SD (n = 8 for both groups). ***p < 0.001 by unpaired t-test.
Taha et al. BMC Neuroscience 2012 13:131 doi:10.1186/1471-2202-13-131