Additional file 1.
Figure S1. Experimental overview. The rationale for the present study was based on our recently published findings [15,16] regarding the in vitro effects of ethanol on mRNA expression in mouse neural stem cells (NSCs), where changes in a considerable portion of genes involved in p53 signaling, cell cycle regulation, apoptosis and DNA damage and repair were observed and independently confirmed using real-time quantitative RT-PCR (qRT-PCR). In the present study, we first examined whether there were similar changes in peripheral blood leukocytes (PBLs) in an in vivo rat binge drinking model using microarray data. Then, based on the considerable overlap and correlated changes, we confirmed several of the rat findings and tested their validity in two sets of human samples: PBLs from subjects with alcohol use disorders, and lymphoblasts (LBs) from a normal human subject. We noted that all four of these data sources showed evidence of dysregulation of the genes of interest, although the specific genes most affected could vary across models. In addition, several of the genes showed highly significant correlations with medical, neuropsychological, neuroimaging, and demographic traits in our human subjects.
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Hicks et al. BMC Neuroscience 2012 13:128 doi:10.1186/1471-2202-13-128