|Sensitivities of primary neurons, SH-SY5Y human neuroblastomas and ESN to the seven classical BoNT serotypes|
|BoNT serotype||Target SNARE protein||Primary neurons EC50(pM)||SH-SY5Y EC50(pM)||ESNs EC50(pM)||95% confidence intervals|
Notes: Primary neurons’ values are compiled from two sources. Annotation by (A) represents 50% inhibition of neurotransmitter release in rat cerebellar neuron cultures exposed to BoNTs for 24 h (a 90% correlation was shown between neurotransmitter release and SNARE integrity) , whereas annotation by (B) represents cleavage of 50% of SNAP-25 protein in fetal mouse spinal cord cultures exposed to BoNT for 48 h . SH-SY5Y neuroblastomas’ values indicate inhibition of 50% of noradrenalin release in SH-SY5Y human neuroblastomas cells after 72 h incubation . ESN values represent 50% cleavage of SNARE proteins after a 24 h tonic intoxication. ESN EC50 values for BoNT/B, /C, /D, /F and /G are from this manuscript, while BoNT/A and /E are from McNutt et al. (2011). nr, not reported.
Hubbard et al.
Hubbard et al. BMC Neuroscience 2012 13:127 doi:10.1186/1471-2202-13-127