ESNs develop large, complex axodendritic arbors by DIV 21. The extent of neuronal network complexity and development was imaged over approximately 9 cm2 at DIV 21 by wide-field fluorescent microscopy. Red, dendritic marker MAP2; green, axonal marker MAP-tau. The apposition of mature dendritic and axon processes is demonstrated as co-localized staining (yellow).
Hubbard et al. BMC Neuroscience 2012 13:127 doi:10.1186/1471-2202-13-127