Open Access Highly Accessed Methodology article

High yield derivation of enriched glutamatergic neurons from suspension-cultured mouse ESCs for neurotoxicology research

Kyle S Hubbard1, Ian M Gut1, Megan E Lyman1, Kaylie M Tuznik1, Mariano T Mesngon2 and Patrick M McNutt1*

Author Affiliations

1 United States Army Medical Research Institute of Chemical Defense, 3100 Ricketts Point Rd, Aberdeen Proving Ground, MD 21010, USA

2 Department of Clinical Investigations, Madigan Army Medical Center, 9040 Jackson Avenue, Fort Lewis, WA 98431, USA

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BMC Neuroscience 2012, 13:127  doi:10.1186/1471-2202-13-127

Published: 24 October 2012

Additional files

Additional file 1: Table S1:

Normalized transcript expression for DIV 14 ESNs. Gene names, average FPKMs, standard deviations and coefficient of variations for DIV 14 ESN samples (n=5).

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Additional file 2: Table S2:

Summary of neurotypic gene expression in DIV 14 ESNs. Representative transcripts involved in neuron function, neurogenesis or neurotoxin function are included. Highlighted sequences represent abundant transcripts, defined as an average reads per kilobase of exon per million mapped sequences (FPKM) exceeding 30.

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Additional file 3: Figure S1:

Representative immunoblots demonstrating dose-dependent proteolysis of target specific SNARE proteins 24 h after exposure.

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