Table 1

Nucleotide sequences of primers used for gene expression study by real-time semiquantitative PCR assays.

Gene

Primer

Sequence


BDNF

Forward

reverse

5'-gtgacagtattagcgagtg-3'

5'-gccttccttcgtgtaacc-3'

GAD65

Forward

reverse

5'-gcccgctataagatgtctc-3'

5'-aatcacactgtctgttcc-3'

GAD67

Forward

Reverse

5'-ggcatcttccactccttc-3'

5'-gacgactcttctcttccag-3'

MBP

Forward

reverse

5'-catccttgactccatcgg-3'

5'-tttcatcttgggtcctctg-3'

NGF

Forward

reverse

5'-gacgactcttctcttccag-3'

5'-cgtggctgtggtcttatctc-3'

PDGFαR

Forward

reverse

5'- ctggtgcctgcctcctac-3'

5'- aactcgctggtcttgaacg-3'

p75

Forward

reverse

5'-agtggcatctctgtggac-3'

5'-ctacctcctcacgcttgg-3'

trk-A

Forward

reverse

5'-tgaatctgtcctccaatgc-3'

5'-tgaagcgtctgtgtatgc-3'

GAPDH

Forward

reverse

5'-ggcaagttcaatggcacagtcaag-3'

5'-acatactcagcaccagcatcacc-3'


Massella et al. BMC Neuroscience 2012 13:12   doi:10.1186/1471-2202-13-12

Open Data