miR-125b accelerates neural differentiation. After transfection, the NS/PCs were maintained in differentiation medium. The expression of the NS/PCs progenitor marker Nestin (A), Sox2 (B), Vinemetin (C) and the neuronal marker Tuj1 (D), Map2 (E) were detected by immunofluorescence. Images were acquired by a microscope using a 20 × objective lens. Bars show mean ± SD. All experiments were repeated three times. * P < 0.05 vs. ctr, ** P < 0.01 vs. ctr. (F) Real-time PCR analysis of Nestin, Sox2, Vinemetin, Tuj1 and Nestin expression in the same differentiation time course 72 h after transfection. GAPDH was included as a loading control. Bars show mean ± SD. All experiments were repeated three times. * P < 0.05 vs. ctr.
Cui et al. BMC Neuroscience 2012 13:116 doi:10.1186/1471-2202-13-116