Efficient transfection of miR-125b in NS/PCs. (A) At 24 post-transfection, transfection efficiency was assayed via fluorescent detection of the 5’-FAM labelled miR-125b mimics in living NS/PCs. The high transfection efficiency was demonstrated using fluorescent microscopy. (B) Real-time PCR was used to assess the efficiency of miR-125b transfection at 72 h after differential transfecton. U6 was included as a loading control.
Cui et al. BMC Neuroscience 2012 13:116 doi:10.1186/1471-2202-13-116