Experimental protocol diagram 1. Eighteen male SD rats were randomly divided into 3 groups(n = 6 each): Sham, CON, EA group. At 24 h after the last EA pretreatment (red arrow), the rats (n = 3) from 3 groups were killed for real-time RT-PCR analysis for Notch1, Notch4, Jag1 and Hes1 genes. At 24 h after reperfusion (yellow arrow), the rats (n = 3) from 3 groups were killed for double immunofluorescent staining to show the NICD immunoreactivity.
Zhao et al. BMC Neuroscience 2012 13:111 doi:10.1186/1471-2202-13-111