Figure 5.

PACOCF3 inhibits the decrease in synaptophysin expression caused by HuPrP106-126 treatment. (A) Primary cortical neurons were either left untreated, or treated with 40 μM HuPrP106-126 for 30 minutes, 1 hour or 24 hours. Some samples were pre-treated with 1 μM PACOCF3 before addition of peptide. The cellular localization of synaptophysin was analyzed by immunofluorescence using anti-synaptophysin (green) antibody. The nuclei were stained with DAPI. Scale bar: 50 μM. (B) Intensity of synaptophysin labelling was measured and normalised to cell number. Data expressed as mean ± S.D. of three experiments, *P < 0.05.

Last et al. BMC Neuroscience 2012 13:106   doi:10.1186/1471-2202-13-106
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