Email updates

Keep up to date with the latest news and content from BMC Neuroscience and BioMed Central.

Open Access Highly Accessed Research article

Inhibition of cytosolic Phospholipase A2 prevents prion peptide-induced neuronal damage and co-localisation with Beta III Tubulin

Victoria Last12*, Alun Williams13 and Dirk Werling1

Author Affiliations

1 Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK

2 Present address: Department of Hematology, Cancer Institute, University College Medical School, London NW3 2PF, UK

3 Present address: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK

For all author emails, please log on.

BMC Neuroscience 2012, 13:106  doi:10.1186/1471-2202-13-106

Published: 28 August 2012

Additional files

Additional file 1:

Figure S1.PMA and A23187 induce cPLA2 activation and subsequent release of arachidonic acid within 30 minutes. Primary cortical neurons were labelled with [3 H]-AA for 24 hours then treated with 1 μM PMA and 5 μM A23187 for 30 minutes. Cells were lysed and levels of [3 H]-AA measured in supernatant and lysates to calculate a% [3 H]-AA release. Data expressed as mean ± S.D. of three experiments. **P < 0.01, ***P < 0.001.

Format: TIFF Size: 75KB Download file

Open Data