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Inhibition of cytosolic Phospholipase A2 prevents prion peptide-induced neuronal damage and co-localisation with Beta III Tubulin

Victoria Last12*, Alun Williams13 and Dirk Werling1

Author Affiliations

1 Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK

2 Present address: Department of Hematology, Cancer Institute, University College Medical School, London NW3 2PF, UK

3 Present address: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK

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BMC Neuroscience 2012, 13:106  doi:10.1186/1471-2202-13-106

Published: 28 August 2012

Additional files

Additional file 1:

Figure S1.PMA and A23187 induce cPLA2 activation and subsequent release of arachidonic acid within 30 minutes. Primary cortical neurons were labelled with [3 H]-AA for 24 hours then treated with 1 μM PMA and 5 μM A23187 for 30 minutes. Cells were lysed and levels of [3 H]-AA measured in supernatant and lysates to calculate a% [3 H]-AA release. Data expressed as mean ± S.D. of three experiments. **P < 0.01, ***P < 0.001.

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