Requirement of screened components for photoreceptor cell development. (A) Bar graph represents frequency of defective photoreceptor axon projections in 3rd instar larval brain. Statistics were determined using the χ2-test with Yate's correction where ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05. (B) Bar graph shows relative rhabdomere length in adult flies with down regulation of tested components. Statistics were determined using a one-tailed Student's t-test where ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05. (C) Photoreceptor axons projection in wild type 3rd instar larval brain visualized with the 24B10 antibody. (D-E) Abnormal R cells growth and termination in 3rd instar larvae with down regulated Chif and Capt (chifBG02820, captRNAi/GMR-Gal4). Arrows indicate irregular axon termination in the lamina plexus, lamina plexus breaks and sites of overgrown axons. (F-K) Exemplary brain sections represent rhabdomere length and morphology in tested mutants in comparison to control (F). Arrows show vacuoles in retina and black bars indicate wt rhabdomere length. (L-O) capt and Cam GFP trap lines show expression patterns for Cam and Capt in the larval brain. (M, O) Enlarged view of eye discs. Arrows indicate expression pattern in optic lobes.
Marrone et al. BMC Neuroscience 2011 12:93 doi:10.1186/1471-2202-12-93