Additional file 2.
Migration of TG WT and TG G93A microglia towards the chemoattractant MCP-1: (A) A representative image of microglial cells on fibronectin-coated coverglass overlaying a Dunn migration chamber with cells facing the chamber. The image was taken on the LEICA Microsystems AF6000LX microscope on a x10 objective, with environmental control to maintain the slide at 37°C. To record migration, images were taken every 3 minutes for 1 hour using Leica software. At the top of the picture is the edge of the outer well, which was filled with medium containing MCP-1. The lower edge is the inner well containing normal medium. Scale bar = 50 μm. (B) An example of analysis of migrated distance represents vector diagrams of cell displacement recorded one hour after setting up the Dunn chamber. Each point represents the position of a cell, which at time 0 is positioned at the intersection of the two axes. The × and Y axes are in μm. Labels: FIB - indicates the coverglass was coated with fibronectin, MCP-1 - monocyte chemoattractant protein-1, 300 - indicates 300 ng/ml concentration of MCP-1.
Format: PNG Size: 183KB Download file
Sargsyan et al. BMC Neuroscience 2011 12:91 doi:10.1186/1471-2202-12-91