Reduced spreading ability of TG G93A microglia. (A) Representative time-lapse frames of a microglial cell spreading on a fibronectin-coated surface are shown as an example. Scale bar = 10 μm. (B) The same frames as in A modified for analysis of cell surface area. The frames are matched with the time (in seconds (s) indicated above arrow) that elapsed from the initiation of recording. (C) Total spreading profile of NTG (WT) and TG WT microglia. Microglia that initiated spreading within first 100 s of recording were classified as "Spreading". Microglia that initiated spreading after the first 100 s of recording were classified as "Delayed". Microglia that did not spread during the entire recording time were classified as "Stationary". (D) Total spreading profile of NTG (G93A) and TG G93A microglia. Data are mean ± SEM; Student's t test, * p = 0.0195 for Spreading and * p = 0.0483 for Stationary TG G93A microglia versus respective NTG (G93A) cells, n = ten TG G93A mice (36 cells) and nine NTG (G93A) mice (30 cells) from four litters, and three TG WT mice (14 cells) and four NTG (WT) mice (14 cells) from two litters.
Sargsyan et al. BMC Neuroscience 2011 12:91 doi:10.1186/1471-2202-12-91