Expression analysis of NFAT isoforms and P2X receptor subunits. RT-PCR expression analysis of undifferentiated PC12-NFAT-Luc cells with PCR primers specific for the indicated P2X receptor (A) or NFAT isoforms (C). Panel B shows positive controls for the P2X receptors that were not detected in undifferentiated PC12-NFAT-Luc cells. For the amplification of P2X6, cDNA from NGF-treated PC12-NFAT-Luc cells used as template. P2X7 was amplified from a plasmid containing mouse P2X7 cDNA. Negative control reactions without DNA template (control) are presented for the primer sets for P2X6 and P2X7 (panel B) and the combined primers sets for the 4 NFAT isoforms (panel C). Migration of size markers (M) is indicated in bp.
Prasai et al. BMC Neuroscience 2011 12:90 doi:10.1186/1471-2202-12-90