MeCP2 binds to the promoter region of two target genes in SH-SY5Y cells. Immunoprecipitation (IP) was performed using an anti-MeCP2 antibody or normal rabbit serum (NRS) as negative control. Equal amounts of precleared chromatin were processed without IP as total input control. The purified DNA was amplified by PCR using primers located within the 1.0 kb upstream genomic regions from the transcriptional start sites of the APBB3, PCDHB1, or PCDH7 genes. SNURF/SNRPN was used as a positive control for a promoter previously demonstrated to bind MeCP2. GAPDH was used as a negative control.
Miyake et al. BMC Neuroscience 2011 12:81 doi:10.1186/1471-2202-12-81