cry gene expression is present in cyc mutants. cyc embryos and their WT siblings were raised in a 14:10 h L/D cycle, fixed at 72 hpf, ZT 0, and then processed for WISH. In both (A-C, G-I) WT embryos and (D-F, J-L) cyc mutants, cry1b and cry3 are expressed in the (A, D, G, J) brain (closed arrowheads), (B, E, H, K) liver (gray arrowheads), and (C, F, I, L) ear (open white arrowheads). Experiment was repeated three times, and representative images are shown. All images are lateral views, anterior to the left. Scale bars = 40 μm for (A-B, D-E, G-H, J-K) and 100 μm for (C, F, I, L).
Noche et al. BMC Neuroscience 2011 12:7 doi:10.1186/1471-2202-12-7