Figure 8.

A-F. Effect of knockdown of Nox-2 gene (Nox-2 siRNA) on the ROS production and NO release in BV-2 cells treated with LPS. Real time RT-PCR quantitative analysis showed that about 75-80% of the endogenous Nox-2 gene expression was inhibited by its siRNA transfection in BV-2 cells (A). Panel B shows the Nox-2 mRNA amplification curve in Nox-2 siRNA treated and untreated BV-2 cells. The flow cytometric analysis shows that LPS did not induce ROS generation significantly in Nox-2 siRNA treated BV-2 cells compared to the LPS-treated non-transfected cells and preincubation of BV-2 cells with Dex further suppressed the induction of ROS production (C, D). Quantitative analysis of flow cytometric results are shown in panel D and E. Comparison between Nox-2 siRNA transfected cells and scrambled treated cells (Negative Control) also showed that LPS was unable to induce ROS production in the cells transfected with Nox-2 siRNA (E). Nitrite assay shows that LPS did not induce NO synthesis in Nox-2 siRNA treated BV-2 cells (F). The data represent the mean ± SD (n = 4) * p < 0.05; ** p < 0.01

Huo et al. BMC Neuroscience 2011 12:49   doi:10.1186/1471-2202-12-49
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