Figure 11.

A-E. Effects of knockdown of MKP-1 gene on ROS production and NO release in BV-2 cells treated with LPS. Real time RT-PCR analysis showed that about 75-80% of the endogenous MKP-1 gene expression was inhibited in microglia after MKP-1 knockdown (MKP-1 siRNA) (A). Panel B shows MKP-1 mRNA amplification curves in MKP-1 siRNA treated cells and control cells. Panels C represents the results of flow cytometric analysis of DCF fluorescence intensity from one sample of each group obtained from three independent experiments performed. Inhibition of MKP-1 gene expression increased the ROS production in MKP-1 siRNA treated BV-2 cells as well as MKP-1 siRNA treated cells treated with LPS or LPS+Dex, compared with untreated BV-2 cells (control) or cells transfected with negative siRNA (negative control) (C, D). Similarly, inhibition of MKP-1 gene expression increased the NO release from LPS alone-treated cells as well as MKP-1 siRNA treated cells treated with LPS or LPS+Dex, compared to that from negative control BV-2 cells (E). The data represent the mean ± SD (n = 4), * p < 0.05; ** p < 0.01

Huo et al. BMC Neuroscience 2011 12:49   doi:10.1186/1471-2202-12-49
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