Figure 10.

A-H. Expression of MKP-1 in BV-2 cells and inhibition of Nox-2 expression and NO release in MKP-1-overexpressed BV-2 cells. Immunofluorescence analysis shows that MKP-1 (red) expression, which was hardly detectable in control cells (A), and cells exposed to Dex alone (B), was induced in cells exposed to LPS for 6 h (C). The induction was further increased when the cells were pre-incubated with Dex (D). Note that the cells were counterstained with DAPI (blue). Panel E shows that MKP-1 mRNA expression fold change is significantly increased in BV-2 cells transfected with MKP (MKP-1+), compared with untreated cells (control). MKP-1 mRNA amplification curves for MKP-1+ and control cells are shown in panel F. Furthermore, real time RT-PCR analysis and nitrite assay showed that MKP-1 overexpression significantly prevented the increase of Nox-2 mRNA expression level (G) and NO release (H) respectively, in MKP-1+ BV-2 cells and MKP-1+ cells treated with LPS. The data represent the mean ± SD (n = 3) * p < 0.05. Scale bar (A-D), 50 μm

Huo et al. BMC Neuroscience 2011 12:49   doi:10.1186/1471-2202-12-49
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