Table 1

Metabolite/5-HT and metabolite/DA ratios in the cortex, hypothalamus, caudate-putamen, midbrain and brainstem/pons of WT and Mecp2-/y.

Brain Area

Group

5HIAA/5HT ratio

(HVA + DOPAC)/DA ratio


Cortex

P35 Wt

1.68 (+/- 0.14)

1.71 (+/- 0.42)


P35 Ko

1.88 (+/- 0.29)

1.33 (+/- 0.43)


P55 Wt

1.50 (+/- 0.17)

0.75 (+/- 0.10)


P55 Ko

1.47 (+/- 0.29)

0.77 (+/- 0.12)


Hypothalamus

P35 Wt

1.92 (+/- 0.26)

0.85 (+/- 0.06)


P35 Ko

2.61 (+/- 0.37) *

0.94 (+/- 0.12)


P55 Wt

1.37 (+/- 0.12)

0.73 (+/- 0.15)


P55 Ko

1.54 (+/- 0.22)

0.62 (+/- 0.09)


CaudatePutamen

P35 Wt

1.91 (+/- 0.11)

0.47 (+/- 0.03)


P35 Ko

2.64 (+/- 0.22) **

0.62 (+/- 0.05)


P55 Wt

1.06 (+/- 0.13)

0.30 (+/- 0.02)


P55 Ko

1.02 (+/- 0.08)

0.33 (+/- 0.02)


Midbrain

P35 Wt

1.51 (+/- 0.23)

1.66 (+/- 0.14)


P35 Ko

1.79 (+/- 0.27)

1.64 (+/- 0.09)


P55 Wt

1.06 (+/- 0.13)

1.07 (+/- 0.13)


P55 Ko

1.02 (+/- 0.08)

0.99 (+/- 0.04)


Brainstem + Pons

P35 Wt

2.27 (+/- 0.39)

0.36 (+/- 0.03)


P35 Ko

2.18 (+/- 0.31)

0.43 (+/- 0.09)


P55 Wt

1.17 (+/- 0.06)

0.44 (+/- 0.06)


P55 Ko

2.00 (+/- 0.51) *

0.39 (+/- 0.02)


Shown are mean values +/- S.E.M. for the ratios of dopaminergic metabolites HVA + DOPAC/DA and serotonergic metabolite 5-HIAA/5-HT in the cortex (Cx), the hypothalamus (Hyp), the caudate-putamen (CPu), midbrain (Md) and brainstem + pons (BP) of Mecp2-/y and their WT littermates at P35 and P55. Dopamine turnover was unafected in the Cx, Hyp, CPu, Md and BP at both P35 and P55 in Mecp2-/y compared to their age-matched WT littermates. Serotonergic turnover was not modified in Cx and Md at any age investigated. However, Hyp and CPu dosage revealed a significant increase of 5-HIAA/5-HT ratio at P35 but not P55. In turn, values from the BP indicate a clear increase in the serotonergic turnover in Mecp2-/y when they exhibit the most severe phenotype (P55). (*p < 0.05, **p < 0.01).

Panayotis et al. BMC Neuroscience 2011 12:47   doi:10.1186/1471-2202-12-47

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