Shear stress inhibits cell proliferation. Panel A: schematic representation of the cell cycle regulation by BTG family proteins. Functional correlation between gene expression and cell cycle was determined using Pathway analysis software. Note how shear stress increased the RNA levels of genes encoding the cell cycle inhibitory effectors BTG1, BTG2 and PMRT1 (Panel B). This finding was also supported by the comparative analysis (flow vs. no-flow) of the expression level of cell cycle modulatory proteins (Panel C). Note that genes and protein related to positive modulators of the cell cycle progression were significantly downregulated. Note also that "*" and "**" refers to a statistically significant (n = 4; p < 0.05) changes (increase and decrease respectively).
Cucullo et al. BMC Neuroscience 2011 12:40 doi:10.1186/1471-2202-12-40